1 2 ChIP - exo interrogation of Crp , DNA , and 3 RNAP holoenzyme interactions 4 5 6
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ChIP - exo interrogation of Crp , DNA , and 3 RNAP holoenzyme interactions 4 5 6
21 Numerous in vitro studies have yielded a refined picture of the structural and molecular 22 associations between Cyclic-AMP receptor protein (Crp), the DNA motif, and RNA polymerase 23 (RNAP) holoenzyme. In this study, high-resolution ChIP-exonuclease (ChIP-exo) was applied to 24 study Crp binding in vivo and at genome-scale. Surprisingly, Crp was found to provide little to 25 no protection ...
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Initiation of transcription is a primary means for controlling gene expression. In bacteria, the RNA polymerase (RNAP) holoenzyme binds and unwinds promoter DNA, forming the transcription bubble of the open promoter complex (RPo). We have determined crystal structures, refined to 4.14 Å-resolution, of RPo containing Thermus aquaticus RNAP holoenzyme and promoter DNA that includes the full trans...
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RNA polymerase (RNAP) is a major target of gene regulation. Thermus thermophilus bacteriophage P23-45 encodes two RNAP binding proteins, gp39 and gp76, which shut off host gene transcription while allowing orderly transcription of phage genes. We previously reported the structure of the T. thermophilus RNAP•σA holoenzyme complexed with gp39. Here, we solved the structure of the RNAP•σA holoenzy...
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تاریخ انتشار 2016